Confocal Laser Scanning Microscopy Laboratory
SMIC maintains two confocal laser scanning microscopes: an Olympus Fluoview 1200 confocal supported by recent funding from the National Science Foundation's Major Research Instrumentation Program, and an Olympus Fluoview 300 confocal supported by a grant from the Alden Trust Foundation.
Olympus Fluoview 300 confocal laser scanning microscope: equipped with Ar- and HeNe-lasers for blue, green and red fluorescence, a 2-channel imaging system, an Olympus BX61 upright microscope with DIC optics and software for image collection and analysis.
Olympus Fluoview 1200 confocal laser scanning microscope: this state-of-the-art confocal microscope is the latest addition to SMIC. For more information, see the Olympus brochure.
- Spectral and filter scan units
- 5 laser channels
- High sensitivity GaAsP Detector for imaging low level fluorescence
- SIM Scanner for simultaneous photostimulation during time-lapse imaging
- Enhanced reliable objectives for live cell imaging
- High-precision focus and Z-tracking through extended time-lapse imaging
- 3D rendering and image analysis with advanced software
- Spectral unmixing for separating similar fluorophores
- Multi-dimensional time-lapse imaging of live cells/organisms
- High-resolution 3D rendering of Z-stack images
- FRET and FRAP technologies
- Multi-laser combiner for simultaneous imaging of up to five different fluorophores
- DIC imaging
- Multi-point, tile and mapping scans
- 3D Mosaic imaging