Professor of Biology in the Department
Other positions held: 1989-1990, Visiting Scientist, John Innes Institute,
Norwich England, 1997-1998, Visiting Professor, Section of Plant Biology,
Courses taught at Skidmore:
Economic Botany (BI 180), Plant Biology (BI 237), Plant Physiology (BI
337), Plant Biotechnology (BI 338), Marine Biology (BI 140), Biological
Electron Microscopy (BI 311), A Green World (LS2)
Domozych is a plant cell biologist who studies the cell walls and
secretory mechanisms in green algae. He is interested in the biochemistry
and cell biology of cell wall components (esp. pectins) and the extracellular
polymeric substances (EPS) especially as they function during the formation
of biofilms in transient wetlands of the Adirondacks. Dr. Domozych's primary
research tools include
light, confocal laser scanning and electron
microscopy, various biochemical techniques and immunocytochemistry.
His research organisms are desmids. Desmids are large and beautiful green
algae that secrete numerous cell wall and EPS macromolecules and use them
in such behaviors as substrate adhesion and gliding. Many desmids also
possess an extensive endomembrane system including more than a hundred
Golgi bodies and thousands of different vesicles. In addition, they have
a spectacular, actin-mediated cytoplasmic streaming mechanism which delivers
the vesicles to specific points of the cell surface. Currently, his research
work is involved with answering questions like:
- How do various EPS macromolecules function in the adhesion,
gliding and ensheathments mechanisms of desmids associated with biofilms?
- How are various extracellular macromolecules processed
in the plant Golgi Apparatus, packaged in specific vesicle types and
released to cytoplasmic streaming mechanism?
- How do particular vesicles get to specific sites on
the surface for selected release?
- What are the signal transduction moleculaes that control
Over the past year, some of the projects
that his research students and he have performed include:
- isolation and characterization of secretory polysaccharides
in desmids and subsequent antibody production.
- immunocytochemical (both light and electron microscopy)
localization of secretory macromolecules within the cell
- the role of calcium and calmodulin on the secretory
- characterization of cytomotile proteins involved in
secretion including actin, profilin and myosin.
- the role of extracellular polymeric compunds in desmid-associated
Dr. Domozych has published over 30 papers. Some recent publications
- Domozych, D.S.,
Roberts, R., Danyow, C., Flitter, R., Smith, B. and Providence, K. (2003)
Plasmolysis, Hechtian strand formation, and localized membrane-wall
adhesion in the desmid, Closterium acerosum (Chlorophyta).
Journal of Phycology 39: 1194-1206.
- Baylson, F.A.,
Stevens, B.W. and Domozych, D.S. (2001) Composition and synthesis of
the pectin and protein components of the cell wall of Closterium
acersoum (Chlorophyta). Journal of Phycology,
- Linde, J., Morse,
L. and Domozych, D.S. (2001) Calmodulin and its role in the secretory
apparatus of the desmid Closterium. International Journal
of Plant Sciences, 162: 15-27.
- Domozych, D.S.
(1999) Disruption of the Golgi apparatus and secretory mechanism in
the desmid, Closterium acerosum, by brefeldin A. Journal
of Expermental Botany, 50: 1323-1330.
- Domozych, D.S.
(1999) Perturbation of the secretory network in Closterium acerosum
by Na+-selective ionophores. Protoplasma, 206: 41-56.
- Lynch, T.M.,
Lintilhac, P.M. and Domozych, D.S. (1998) Mechanotransduction molecules
in the plant gravisensory response: amyloplast/statolith membranes contain
a §1-integrin-like protein. Protoplasma, 201: 92-100.