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Skidmore College
Institutional Biosafety Committee

What Must Be Registered

Experiments involving the following must be registered with the IBC:

  • Pathogens affecting humans, animals or plants
  • Materials potentially containing human pathogens (for example, unfixed human specimens, human blood)
  • Recombinant DNA molecules including virus vectors
  • Work that does not qualify under NIH Guidelines Appendix CI-IX
  • Human cell lines that are not well-characterized or require Risk Group 2 containment
  • Generation of de novo transgenic animals, defined as the addition of foreign DNA or subtraction of a portion of the animal genome using recombinant DNA technology. The breeding of transgenic animals to generate additional transgenic offspring does not require IBC approval. Those transgenic animals that already exist or which have been purchased also do not require IBC approval. 
  • All research involving the use of recombinant or synthetic nucleic acid molecules containing no more than two-thirds of the genome of any eukaryotic virus, or biohazards
  • Animal Subjects: All research involving the use of recombinant molecules or biohazards in whole animals requires both IBC and IACUC approval
  • Human Subjects: Any research involving the introduction of recombinant molecules or biohazards into human subjects must be approved by the IBC and by the IRB.


Human Cells and Tissues

Human and non-human primate cells should be handled using Risk Group 2 (RG-2) practices and containment. All work should be performed in a biosafety cabinet and all material should be decontaminated by autoclaving or disinfection before discarding. Appropriate training in the handling of blood-borne pathogens and up-to-date hepatitis B vaccinations may be required.

Select Agents and Toxins

Select agents are specific pathogens and toxins that have the potential to pose a severe threat to public health and safety as defined by the USA PATRIOT Act and the Public Health Security and Bioterrorism Preparedness and Response Act of 2002. The institution must be registered with the CDC and/or USDA before these materials are obtained, used or stored.


Risk Group One (RG-1)

Risk Group One (RG-1) agents are usually not placed on a list but include all microorganisms that do not pose a health risk to healthy adult humans. It must not be assumed that an organism not listed as a RG 2, 3, or 4 agent is an RG-1 agent; emerging or unknown organisms should be treated as biohazardous until research proves otherwise. Examples of agents in RG-1 include Bacillus subtilus, infectious canine hepatitis viruses, influenza reference strains A/PR/8/34, A/WS/33, Escherichia coli K12, Saccharomyces cerevisiae and other agents listen in Appendix C-II of the NIH Guidelines for Research Involving Recombinant DNA Molecules and other vectors such as Baculovirus.

In addition to the examples listed above, the following low-risk oncogenic viruses have been identified as RG-1 agents:

Adenvirus 7-Simian virus 40 (Ad7-SV40)
Avian Leucosis virus 
Bovine leukemia virus
Bovine papilloma virus
Chick-embryo-lethan orphan (CELO) virus
Dog sarcoma virus
Guinea pig herpes virus
Lucke (frog) virus
Hamster leukemia virus
Marek’s disease virus
Mason-Pfizer monkey virus
Mouse mammary tumor virus
Murine leukemia virus
Murine sarcoma virus
Polyoma virus
Rat leukemia virus
Rous sarcoma virus
Shope vibroma virus
Shope papilloma virus
Simian virus 40 (SV-40)


Risk Group Two (RG-2)

RG-2 agents are of moderate potential hazard to healthy adult humans and the environment. Such agents may produce disease of varying degrees of severity from accidental inoculation, injection or other means of cutaneous penetration but can usually be adequately and safely contained by ordinary laboratory techniques. Some agents may cause disease by contact or respiratory routes, but they are self-limiting and do not cause a serious illness, such as the cause of the common cold, the rhinoviruses. The following organisms have been identified as RG-2 agents:

Acinetobacter baumannii
Actinobacillus spp 
Actinomyces pyogenes
Aeromonas hydrophila
Amycolata autotrophica
Archanobacterium haemolyticum
Bacteroides spp.
Borrelia recurrentis
Burkholderia (except those in RG-3)
Campylobacter coli
Clostridium chauvoei
Corynebacterium diphtheria
Dermatophilus congolensis
Edwardsiella tarda
Erysipelothrix rhusiopathiae
Escherichia coli
Haemophilus ducreyi
Helicobacter pylori
Klebsiella spp.
Leptospira interrogans
Listeria spp.
Moraxella spp.
Mycobacterium spp.
Nocardia asteroids
Pasteurella spp.
Plesiomonas shigelloides
Proteus spp.
Salmonella spp.
Sphaerophorus necrophorus
Staphylococcus aureus
Streptobacillus moniliformis
Streptococcus spp
Treponema pallidum
Vibrio cholera
Yersinia enterocolitica
Fungal Agents

Blastomyces dermatitidis
Cladosproium bantianum
Cryptococcus neoformans
Epidermophyton spp.
Exophiala dermatitidis
Fonsecaea pedrosoi
Microsporum spp.
Ochroconis gallopavum
Paracoccidiodes brasilensis
Penicillium marneffei
Sporothrix schenckii
Trichophyton spp.

Parasitic Agents

Ancylostoma spp.
Ascaris spp.
Babesia spp.
Brugia spp.
Coccidian spp.
Cryptosporidium spp.
Cysticercus cellulosae
Echinococcus spp.
Entamoeba histolyitca
Enterobius spp. 
Fasciola spp.
Giardia spp.
Heterophyes spp.
Hymenolepis spp.
Isospora spp.
Leishmania spp.
Loa loa filarial
Microsporidium spp.
Naegleria fowleri
Necator spp.
Onchoerca spp.
Plasmodium spp.
Sarcocystis spp.
Schistosoma spp.
Strongyloides spp.
Taenia solium
Toxocara spp.
Trichinella spiralis
Trypanosoma spp.
Wuchereria bancrofti


Adenovirus 2
Coxsackie A and B viruses
Parainfluenza virus
Rhinoviruses, all types
Feline sarcoma virus (FeLV)
Gibbon leukemia virus
Hepatitis A, D, E
Influenza viruses
Lymphogranuloma venereum agent
Measles virus
Molluscum contagiosum virus
Rubella virus
Paravaccina virus
Respiratory syncytial virus