Operating Procedures and Techniques 

Operating Procedures for the following microscopes:

Olympus BX60 Upright Fluorescent Light Microscope

Olympus BX63 upright Florescent Light Microscope with time laps

Olympus IX70 Inverted Fluorescent Light Microscope

Olympus IX83 Inverted Fluorescent Light Microscope with time laps

Olympus SZX-12 Stereomicroscope

Fluoview 1000 Confocal Laser Scanning Microscope

FEI Quattro S Field Emission Scanning Electron Microscope

FEI Quattro S Field Emission Scanning Electron Microscope (remote access)

FEI Quattro S FSEM-EDS (Pathfinder Alpine level)

Hitachi HT7800 Transmission Electron Microscope

Hitachi HT7800 Transmission Electron Microscope (remote access)

Ultracut E Ultramicrotome (old microtome)

Leica EM UC6 Microtome (new microtome)

Techniques and Protocols:

FEI Quattro S Cooling Stage Instruction

Cryo Stage Low Vacuum SEM

TEM Tomography Instruction

Coating TEM Grids

Grid Staining

Glass Knife Making

Sputter Coating

Carbon coater procedure

TEM Fixation Protocol

Gene Gun Operating Protocol

Critical Point Dryer