Microscopy-based Research

Many Skidmore faculty members are engaging in microscopy-based research utilizing SMMIC.  For more information, see their individual webpages below.  If you are student and would like to learn more about microscopy or use the microscopy center for your independent research, contact your research advisor, or lily Kozel

Dr. David Domozych, Professor, Biology Department, SMIC Director: plant cell biology, plant evolution

Dr. Janson Breves, Associate Professor, Biology Department: Study on vertebrate endocrine systems of a variety of fish models (e.g., Mozambique tilapia, Atlantic salmon, mummichog, and zebrafish)

Dr. Jennifer Bonner, Associate Professor, Biology Department: Neurobiology of the spinal cord using zebra fish as a model organism

Dr. Sarita Lagalwar, Associate Professor, Neuroscience: Research on neurodegenerative diseases using mouse and human models

Dr. Javier Perez-Moreno, Associate Professor, Physics Department: Polarized light microscopy, photonic band gap engineering research

Dr. Sylvia Franke-McDevitt, Associate Professor, Biology Department: Research on microbial metal homeostasis

 

Recent Publications from SMIC Users

LoRicco J. G., Kozel L., Bagdan K., Epstein R., Domozych D.S. (2023) Endosidin 5 disruption of the Golgi apparatus and extracellular matrix secretion in the unicellular charophyte Penium margaritaceum, Annals of Botany, Volume 131, Issue 6, Pages 967–983, https://doi.org/10.1093/aob/mcad054

Domozych, D.S, LoRicco J. G.(2023) The extracellular matrix of green algae. Plant Physiology, doi:10.1093/plphys/kiad384

Laboy JT, Bonner J, Norman KR. (2023) DEC-7/SUSD2, a sushi domain-containing protein, regulates an ultradian behavior mediated by intestinal epithelial Ca2+ oscillations in Caenorhabditis elegans. Am J Physiol Cell Physiol. 324(5):C1158-C1170. doi: 10.1152/ajpcell.00552.2022. Epub 2023 Apr 17.PMID: 37067458

Domozych, D.S., Bagdan K. (2022) The cell biology of charophytes: Exploring the past and models for the future. Plant Physiology, 190:1588-1608. doi: 10.1093/plphys/kiac390

Lagalwar, S. (2022) Mechanisms of tunneling nanotube-based propagation of neurodegenerative disease proteins.  Frontiers of Molecular Neuroscience, doi: 10.3389/fnmol.2022.957067

Domozych D.S., Kozel L., Lopez K.P. (2021) The effects of osmotic stress on the cell wall-plasma membrane
domains of the unicellular streptophyte, Penium margaritaceum. Protoplasma 258: 1231–1249

Breves J.P., Nelson N.N., Koltenyuk V., Petro-Sakuma C.K., Celino-Brady F.T., Seale A.P. (2021) Enhanced expression of ncc1 and clc2c in the kidney and urinary bladder accompanies freshwater acclimation in Mozambique tilapia. Comparative Biochemistry and Physiology, Part A 260: 111021.

Huang H., Toker N., Burr E., Okoro J., Moog M., Hearing C., Lagalwar S. (2021) Intercellular Propagation and Aggregate Seeding of Mutant Ataxin-1. J Mol Neurosci  https://doi.org/10.1007/s12031-021-01944-1

Domozych D.S., Sun L., Lopez K.P., Reed R., Jeon S., Li M., Jiao C., Sørensen I., Fei Z. and Rose J.K. (2020) Endomembrane architecture and dynamics during secretion of the extracellular matrix of the unicellular charophyte, Penium margaritaceum. Journal of Experimental Botany, Volume 71, Issue 11, 3323–3339

Lopez K.P., Sun L., Reed R., Kang E., Sørensen I., RoseJ.K. and Domozych D.S. (2020) Experimental Manipulation
of Pectin Architecture in the Cell Wall of the Unicellular Charophyte, Penium Margaritaceum. Frontiers in Plant Science, Volume 11, Article 1032

Jiao C., Sørensen I., Sun X., Sun H., BeharH., Alseekh S., Philippe G.,Lopez K.P., Sun L., Reed R., Jeon S., Kiyonami R., Zhang S., Fernie A.R., Brumer H., Domozych D.S., Fei Z., Rose J.K. (2020) The Penium margaritaceum Genome:Hallmarks of the Origins of Land Plants. Cell 181, 1–15

Breves J.P.,Starling J.A., Popovski C.M..Doud J.M.,Tipsmark C.K.,Salinity‑dependent expression of ncc2 in opercular epithelium and gill of mummichog (Fundulus heteroclitus),Journal of Comparative Physiology B (2020) 190, 219–230

Sarasija S, Laboy JT, Ashkavand Z, Bonner J, Tang Y, Norman KR. (2018) Presenilin mutations deregulate mitochondrial Ca2+ homeostasis and metabolic activity causing neurodegeneration in Caenorhabditis elegans. Elife. 2018 Jul 10;7:e33052. doi: 10.7554/eLife.33052.PMID: 29989545

Pérez Ortiz JM, Mollema N, Toker N, Adamski CJ, O'Callaghan B, Duvick L, Friedrich J, Walters MA, Strasser J, Hawkinson JE, Zoghbi HY, Henzler C, Orr HT, Lagalwar S. (2018) Reduction of protein kinase A-mediated phosphorylation of ATXN1-S776 in Purkinje cells delays onset of Ataxia in a SCA1 mouse model. Neurobiol Dis. 116:93-105. doi: 10.1016/j.nbd.2018.05.002.

Ferro A, Carbone E, Zhang J, Marzouk E, Villegas M, Siegel A, Nguyen D, Possidente T, Hartman J, Polley K, Ingram MA, Berry G, Reynolds TH, Possidente B, Frederick K, Ives S, Lagalwar S..(2017) Short-term succinic acid treatment mitigates cerebellar mitochondrial OXPHOS dysfunction, neurodegeneration and ataxia in a Purkinje-specific spinocerebellar ataxia type 1 (SCA1) mouse model. PLoS One. Dec 6;12(12):e0188425. doi: 10.1371/journal.pone.0188425. eCollection 2017

Ferro, A., Carbone, E., Marzouk, E., Siegel, A., Nguyen, D., Polley, K., Hartman,J., Frederick K., Ives, S., Lagalwar, S.. (2017) Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function. J. Vis.Exp. (119), e53758, doi:10.3791/53758. 

Uluisik S., Chapman N.H., Smith R., Poole M., Adams G., Gillis R.B., Besong T.M., Sheldon J., Stiegelmeyer S., Perez L., Samsulrizal N., Wang D., Fisk I.D., Yang N., Baxter C., Rickett D., Fray R., Blanco-Ulate B., Powell A.L., Harding S.E., Craigon J., Rose J.K., Fich E.A., Sun L., Domozych D.S., Fraser P.D., Tucker G.A., Grierson D., Seymour G.B. (2016) Genetic improvement of tomato by targeted control of fruit softening. Nature Biotechnology 34 (9):950-952

Domozych, D. S. (2016) Biosynthesis of the Cell Walls of the Algae. In: The Physiology of Microalgae (ed: Borowitzka, M.A.., Beardall, J. and Raven, J.A.), Springer International; Switzerland, pp. 47-63.

Domozych, D. S. (2015) Cell wall evolution and diversity. In: Polysaccharides (ed: K.G. Ramawat, J.-M. Meriilon), Springer International; Switzerland, pp. 53- 79.

Brocke, R., Fatka, O., Lindemann, R.H., Schindler, E., and Ver Straeten, C.V., 2015, Palynology, dacryconarids and the lower Middle Devonian Basal Choteč Event: Case studies from the Prague and Appalachian basins. In Becker, R.T., P. Konigshof, and C.E. Brett, Eds., Devonian Climate, Sea-Level and Evolutionary Events, Geological Society of London, Special Publications, v. 423.

Domozych, D. S. and Domozych, C. E. (2014) Multicellularity in green algae: upsizing in a walled complex. Frontiers in Plant Science (doi: 10.3389/pls.2014). 

Domozych, D. S. (2014) Penium margaritaceum: a unicellular model organism for studying plant cell wall architecture and dynamics" Plants 3: 543-558. 

Domozych, D. S. Sørensen, I., Popper, Z. A., Ochs, J., Andreas, A., Fangel, J. U., Pielach, A., Sacks, C., Brechka, H., Willats, W. G. T. and Rose, J. K. C. (2014) Pectin metabolism and assembly in the cell gall of the Charophyte Green alga Penium margaritaceum. Plant Physiology 165: 105-118. 

Domozych, D. S., Sørensen, I., Sacks, C., Brechka, H., Andreas, A., Fangel, J. U., Rose, J. K. C.  Willats, W. G. T. and Popper, Z. A. (2014) Disruption of the microtubule network alters cellulose deposition and causes major changes in pectin distribution in the cell wall of the green alga, Penium margaritaceum. Journal of Experimental Botany 65: 465-479. 

Sørensen, I. Fei, Z., Andreas, A., Willats, W. G. T., Domozych, D. S. and Rose, J. K. C. (2014) Stable transformation and reverse genetic analysis of Penium margaritaceum: A platform for studies of Charophycean Green Algae, the immediate ancestors of land plants. Plant Journal 77: 339–351. 

Ochs, J., LaRue, T., Tinaz, B., Yongue, C. and Domozych, D. S. (2014) The cortical cytoskeletal network and cell-wall dynamics in the unicellular charophycean green alga Penium margaritaceum. Annals of Botany 114: 11237-1250. 

Larson, E. R., Domozych, D. S. and Tierney, M. L. (2014) SNARE VTI13 plays a unique role in endosomal trafficking pathways with the vacuole and is essential for cell wall organization and root hair growth in Arabidopsis. Annals of Botany 114: 11147-1150. 

Pielach, A., Leroux, O., Domozych, D.S., Knox, J.P. and Popper, Z. A. (2014) Arabinogalactan protein-rich cell walls, paramural deposits and ergastic globules define the hyaline bodies of rhinanthoid Orobanchaceae haustoria. Annals of Botany 114: 1359-1376. 

Domozych, D. S., Fujimoto, C. and LaRue, T. (2013) Polar expansion dynamics in the plant kingdom: a diverse and multifunctional journey on the path to pollen tubes. Plants 2: 148-173.


Buda, G.J., Barnes, W.J., Fich, E.A., Park, S., Yeats, T.H., Zhao, L., Domozych, D.S. and Rose, J.K.C. (2013) An ATP binding cassette transporter is required for cuticular wax deposition and desiccation tolerance in the moss Physcomitrella patens. Plant Cell 25: 4000-4013.

Ulvskov, P., Paiva, D.S., Domozych D.S., and Harholt, J. (2013) Classification, naming and evolutionary history of glycosyltransferases from sequenced green and red algal genomes. PLOS ONE 8: e76511

Domozych, D.S., Ciancia, M., Fangel, J.U., Mikkelsen, M.D., Ulvskov, P. and Willats, W.G.T. (2012) The cell walls of green algae: a journey through evolution and diversity. Frontiers in Plant Physiology doi: 10.3389/fpls.2012.00082.

Domozych, D.S. (2012) The quest for four-dimensional imaging in plant cell biology: it’s just a matter of time. Annals of Botany 110: 461-474.

Yeats, T. H., Martin, L. B. B., Viart, H., Isaacson, T., He, Y., Zhao, L., Matas, A.J., Buda, G. J., Domozych, D.S., Clausen, M. H. and Rose, J. K. C. (2012) The identification of cutin synthase: formation of the plant polyester cutin. Nature Chemical Biology doi:10.1038/nchembio.960

Sørensen, I., Rose, J. K. C., Doyle, J. J., Domozych, D. S., and Willats, W.G.T. (2012). The Charophycean green algae as model systems to study plant cell walls and other evolutionary adaptations that gave rise to land plant. Plant Signaling and Behavior 7: 1–3.

Sørensen, I., Pettolino, F., Bacic, A., Ralph J., Lu, F., O'Neill, M., Fei, Z. Rose, J.K.C., Domozych, D.S. and Willats, W.G.T. (2011) The Charophycean green algae provide insights into the early origins of plant cell walls. Plant Journal 68, 201-211.

Domozych, D.S., Brechka, H., Britton, A. and Toso, M. (2011) Cell Wall Growth and Modulation Dynamics in a Model Unicellular Green Alga—Penium margaritaceum: Live Cell Labeling with Monoclonal Antibodies. Journal of Botany doi:10.1155/2011/632165.

Popper, Z.A., Michel, G., Hervé, C., Domozych, D.S., Willats, W.G.T., Tuohy, M.G., Kloareg, B. and Stengel, D.B. (2011) Evolution and Diversity of Plant Cell Walls: From Algae to Flowering Plants. Annual Review of Plant Biology 62, 567-590.

Beck A., Watt, R. and Bonner J. (2013) Mounting of zebrafish embryos for optimal microscopy.  Journal of Visual Experimentation (84), e50703, doi:10.3791/50703  Online Video

Ross A., Bonner J. (2012) Activation of Wnt signaling using Lithium Chloride:  An Inquiry-based Model of Undergraduate Laboratory Exercises. Zebrafish 9(4):220-5.
 
Spooner P.M., Bonner J., Maricq A.V., Benian G.M., Norman K.R. (2012) Large isoforms of UNC-89 obscurin are required for muscle cell architecture and calcium release in Caenorhabditis elegans.  PLoS One 7(7):e40182.
 
Bonner J., Letko M., Nikolaus O.B., Krug L., Cooper A., Chadwick B., Conklin P., Lim A., Chien C-B, Dorsky R.I. (2012) Midline crossing is not required for subsequent pathfinding decisions in commissural neurons. Neural Development 7(1):18.
 
Gribble S.L., Kim H.S., Bonner J., Wang X., Dorsky R.I.  (2009) Tcf3 inhibits spinal cord neurogenesis by regulating sox4a expression.  Development 136(5):781-9.
 
Bonner J., Gribble S.L., Veien E.S., Nikolaus O.B., Weidinger G., Dorsky R.I.  (2008) Proliferation and patterning are mediated independently in the dorsal spinal cord downstream of canonical Wnt signaling.  Developmental Biology 313(1):398-407.

Underlined= Skidmore Students or researchers

Bold= Skidmore Faculty