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Skidmore College
Skidmore Analytical Interdisciplinary Laboratory (SAIL)

High performance liquid chromatography (HPLC)

SAIL houses two high-performance liquid chromatographs for the separation and analysis of small molecules in solution.

Dionex UltiMate 3000 HPLC

PerkinElmer Flexar HPLC

Dionex UltiMate 3000 (Chemistry)

  • Quaternary pump
  • Auto-sampler injection
  • Sample and column heaters
  • Isocratic and gradient separations
  • UV-Vis detector monitors up to 4 wavelengths simultaneously
  • 3-D spectra

PerkinElmer Flexar (SAIL)   

  • Binary pump
  • Manual injection
  • UV-Vis detector




Available columns

  • Restek Ultra C18 5-micron 100 x 4.6 mm
  • Restek Ultra C18 5-micron 150 x 4.6 mm
  • Restek Ultra AQ C18 5-micron 150 x 4.6 mm
  • Shodex KC-811 ion exchange column

Recent applications

  • Determination of caffeine content of various beverages
  • Identification of artificial dyes in foods
  • Organic and analytical chemistry courses


Dionex UltiMate 3000 Standard Operating Procedure

PerkinElmer Flexar Standard Operating Procedure