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Skidmore College
Skidmore Microscopy Imaging Center - SMIC

Confocal Laser Scanning Microscopy Laboratory 

SMIC maintains two confocal laser scanning microscopes: an Olympus Fluoview 1200 confocal supported by recent funding from the National Science Foundation's Major Research Instrumentation Program, and an Olympus Fluoview 300 confocal supported by a grant from the Alden Trust Foundation.

Equipment

Olympus Fluoview 300 confocal laser scanning microscope: equipped with Ar- and HeNe-lasers for blue, green and red fluorescence, a 2-channel imaging system, an Olympus BX61 upright microscope with DIC optics and software for image collection and analysis. 

Olympus Fluoview 1200 confocal laser scanning microscope: this state-of-the-art confocal microscope is the latest addition to SMIC.  For more information, see the Olympus brochure.

  • Specifications:
    • Spectral and filter scan units
    • 5 laser channels
    • High sensitivity GaAsP Detector for imaging low level fluorescence
    • SIM Scanner for simultaneous photostimulation during time-lapse imaging
    • Enhanced reliable objectives for live cell imaging
    • High-precision focus and Z-tracking through extended time-lapse imaging
    • 3D rendering and image analysis with advanced software
    • Spectral unmixing for separating similar fluorophores

  • Applications:
    • Multi-dimensional time-lapse imaging of live cells/organisms
    • High-resolution 3D rendering of Z-stack images
    • FRET and FRAP technologies
    • Multi-laser combiner for simultaneous imaging of up to five different fluorophores
    • DIC imaging
    • Multi-point, tile and mapping scans
    • 3D Mosaic imaging

Confocal Image Gallery

Facilities